Optimized transgenesis inXenopus laevis/gilliisogenetic clones for immunological studies

Transgenesis-Mediated Reproductive Dysfunction and Tumorigenesis: Effects of Immunological Neutralization

Human chorionic gonadotropin (hCG) was initially thought to be made only during pregnancy, but is now known to also be synthesized by a variety of cancers and is associated with poor patient prognosis. Transgenic expression of βhCG in mice causes hyper-luteinized ovaries, a loss in estrous cyclicity and infertility, increased body weight, prolactinomas and mammary gland tumors. Strategies were ...

An optimized transgenesis system for Drosophila using germ-line-specific C31 integrases

Germ-line transformation via transposable elements is a powerful tool to study gene function in Drosophila melanogaster. However, some inherent characteristics of transposon-mediated transgenesis limit its use for transgene analysis. Here, we circumvent these limitations by optimizing a C31-based integration system. We generated a collection of lines with precisely mapped attP sites that allow ...

An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases.

Germ-line transformation via transposable elements is a powerful tool to study gene function in Drosophila melanogaster. However, some inherent characteristics of transposon-mediated transgenesis limit its use for transgene analysis. Here, we circumvent these limitations by optimizing a phiC31-based integration system. We generated a collection of lines with precisely mapped attP sites that all...

Immunological Studies in Pneumonia

The serum derived from human cases of pneumonia has been used to sensitize guinea pigs passively. As antigen an autolysate of a pneumococcus culture was employed. By means of Dale's method it has been possible to show with considerable regularity that the blood contains sensitizing antibodies during the course of pneumonia, but none after the crisis. Patients suffering from other diseases have ...

[Immunological studies on cryoglobulin].